週次 |
日期 |
單元主題 |
第1週 |
2/26 |
Overview |
第2週 |
3/05 |
Generation of Targets for Spotted Microarrays (Inoculate, miniprep and DNA gel electrophoresis) |
第3週 |
3/12 |
Generation of Targets for Spotted Microarrays (PCR and spectrophotometer) |
第4週 |
3/19 |
Generation of Targets for Spotted Microarrays (Plate transfer, Beckman FX) |
第5週 |
3/26 |
Printing Spotted Glass Microarrays (Printing design (OmniGrid arrayer)) |
第6週 |
4/02 |
Spring break (no class) |
第7週 |
4/09 |
Printing Spotted Glass Microarrays (Post-processing) |
第8週 |
4/16 |
No class (out for a conference) |
第9週 |
4/23 |
Midterm (No class) |
第10週 |
4/30 |
Expression Analysis of RNA (Cell culture and RNA extraction) Class starts at 1:30. |
第11週 |
5/07 |
Expression Analysis of RNA (RNA purification and quality check) |
第12週 |
5/14 |
Expression Analysis of RNA (cDNA labelling) |
第13週 |
5/21 |
Expression Analysis of RNA (microarray hybridization) (No class) |
第14週 |
5/28 |
Microarray Bioinformatics (Gal file preparation and Scan image (Axon scanner intro)) (Class starts at 2:00) |
第15週 |
6/04 |
Microarray Bioinformatics (Web-base microarray analysis and IPA introduction) |
第16週 |
6/11 |
Microarray Bioinformatics (Real time RT PCR) |
第17週 |
6/18 |
Microarray Bioinformatics (Illumina and Affymetrix system introduction) |
第18週 |
6/25 |
Final exam |